Figure 5.

Effect of GILZ and DUSP1 GBS1 deletion in A549 cells. (A) GR ChIP-seq tag density for dex-treated (top) A549 cells and U2OS-GR18 cells (bottom) for a promoter region of the GR target gene GILZ. (B) Same as for (A) except that for the DUSP1 gene. (C) Relative mRNA expression levels as determined by qPCR for the FKBP5, GILZ and DUSP1 genes are shown for wt A549 (n = 6), clonal A549 lines with deleted GILZ GBS1 (n = 5) and for clonal lines with deleted DUSP1 GBS1 (n = 5). Circles indicate values for each individual clonal line. Horizontal lines and error bars: Averages ± SEM for cells treated with vehicle or 1 μM dex overnight. Unpaired two-sided Mann–Whitney U test: n.s. not significant, *P < 0.05, **P < 0.01, ***P < 0.001.