Figure 8.

Model depicting how cell type-specific long-range interactions and enhancer-blocking can direct the activity of an enhancer shared between cell types to distinct transcripts. Top: An A549-specific long-range DNA-looping interaction between GBS1 and TSS1 enables GR-dependent regulation of GILZ transcript variant 1. Simultaneously, enhancer-blocking insulates GILZ transcript variants 2 and 3 from the regulatory activity of GBS1. Bottom: In U2OS-GR18 cells the absence of both looping and enhancer-blocking facilitates GR-dependent regulation of the nearby transcript variants 2 and 3.