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Analysis of Ca2+-dependent CaM binding to the BCA1p N terminus. The N-terminal region of BCA1p (amino acids 1–157) was expressed in E. coli as a fusion protein with MBP. Factor Xa-treated (+) or non-treated (−) fusion protein was analyzed by SDS-PAGE and Coomassie staining (left). The same fractions were blotted to PVDF membrane and overlaid with 125I-CaM in the presence or absence of Ca2+ (middle), or were probed with anti-BCA1p (right).
