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Elution profiles from reverse-phase HPLC of the phosphorylation reaction after different times of phosphorylation of synthetic peptide Ala-19 to Met-39 (see “Materials and Methods” for details). Peaks 1, 2, and 3 eluted at 21%, 22%, and 20% acetonitrile, respectively. An asterisk indicates that the peak was radioactive when [γ-32P]ATP was used in the phosphorylation assay.
