Figure 6.

Levels of Smad2 phosphorylation were upregulated after Ang-II exposure, indicative of TGF-β activity. (a) Representative western blot images and semi-quantification using densitometry of pSmad2 and total Smad2/3 protein levels relative to actin at six hours. (b) Densitometry was used for semi-quantification of pSmad2 levels relative to total Smad2/3 protein levels. (c) Representative western blot images and semi-quantification using densitometry of pSmad2 and total Smad2/3 protein levels relative to actin at 24 hours. (d) Densitometry was used for semi-quantification of pSmad2 levels relative to total Smad2/3 protein levels.

Data are expressed as means ± SEM and normalized to actin.

n = 5–6.

*p < 0.05 and **p < 0.01, compared to saline control.

Ang-II: Angiotensin II; PBS: phosphate-buffered saline; pSMAD2: phosphorylated Smad2; Ser: serine.