Figure 5.

Expression of expansin (LeExp2), XET (LeEXT1), and EGase (Cel7) genes during tomato fruit development. Flowers were tagged at anthesis and fruit diameter measured over a period of 50 d. A regression curve was established from data obtained with 15 fruit. Poly(A⁺) RNA-blot analysis of LeExp2, LeEXT1, and Cel7 expression was done during tomato fruit development. Northern blots (1 μg per lane) were hybridized successively with the LeExp2, LeEXT1, Cel7, and actin cDNA probes. (I, 0.5- to 1.0-cm diameter fruit; II, 2- to 3-cm diameter fruit; III, 4- to 6-cm diameter fruit; IG, immature green; MG, mature green; Br, breaker; Tu, turning; Pi, pink; LR, light red; RR, red-ripe). RNA gel blots were hybridized and washed at the same stringency as in Figure 2.