Fig. 2.

Cold induction and subcellular localization of AQUILO (AQ) in grapevine. (A) Expression pattern of VaAQ and VvAQ under cold stress in shoot apices and young leaves. MALATE DEHYDROGENASE (MDH, VIT_07s0005g03350) and ACTIN (VIT_04s0044g00580) genes were used as calibration qRT-PCR controls. Each reaction was performed in triplicate (technical replicates) with three independent biological replicates (n=3). The relative expression was calculated using the 2^−ΔΔCt method. Error bars represent the SE. Different letters indicate significant differences at a given time point (P<0.05; one-way ANOVA). (B) Subcellular localization of VaAQ in Arabidopsis protoplasts. The fluorescent signal generated by a 35S:VaAQ-GFP construct transfected in Arabidopsis protoplasts was detected by confocal laser scanning microscopy. The images are representative of three independent experiments resulting in the same fluorescence pattern. Scale bars correspond to 10 µm.