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. 2018 Jan 29;69(7):1749–1764. doi: 10.1093/jxb/ery020

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© The Author(s) 2018. Published by Oxford University Press on behalf of the Society for Experimental Biology.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 4. — Effect of VaAQUILO (VaAQ) overexpression (OE) on antioxidant enzyme activities and the expression of ROS-scavenging-related genes. Superoxide dismutase (SOD; A), peroxidase (POD; B), and catalase (CAT; C) activities in OE lines and WT plants under non-stress and cold stress conditions. Gene expression levels of AtFSD2 (At5g51100; D), AtCSD2 (At2g28190; E), AtCAT2 (At4g35090; F), AtAPX1 (At1g07890; G), AtSAPX (At4g08390; H), and AtGPX7 (At4g31870; I) were monitored under the same conditions. Transcript levels were analysed by qRT-PCR using ACTIN2 (ACT2, AT3G18780) and UBIQUITIN10 (UBQ10, At4g05320) as calibration controls. Gene expression was normalized to the expression obtained in the WT under non-stressed conditions. Each reaction was performed in triplicate (technical replicates). Data are mean values ±SE of three biological replicates (n=3). Different letters indicate significant differences compared with the WT under non-stress conditions (P<0.05, as determined by one-way ANOVA with a post-hoc LSD test).