Fig. 5.
The effect of GA3 on HC-triggered bud break. (A and B) GA buds received a single treatment of 1, 5, or 10 ppm GA3. HC–GA buds were treated with the above GA concentrations concomitantly with HC (3% Dormex®). All solutions were formulated in 0.02% Triton X-100, which served as control treatment. All other details are as in Fig. 1. Bud break was recorded 11, 14, 18, 21, and 25 d after treatment. Values represent average bud break of nine groups of 10 cuttings ±SE. (C) GA1 levels were determined in control and HC-treated buds sampled at 48 h and 96 h after treatment as described in Fig. 2. Bars represent means ±SE of three biological repeats (10 buds per repeat). Data points with different letters indicate significantly different values (P<0.05) according to Tukey’s HSD test. Asterisks between treatments indicate significant differences according to Student’s t-test (*P<0.05).