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Fraxetin content in Nicotiana benthamiana leaves. Transient expression was carried out using Agrobacterium tumefaciens transformed with the pBIN empty vector (control), pBIN:S8H, pBIN:F6ʹH2, and simultaneously with vectors encoding F6ʹH2 and S8H. Fraxetin content was quantified using UHPLC. Data are presented as mean ±SD. *P<0.05.
