Fig. 5.

Phenotypic characterization of 6-week-old wild-type (Col-0) plants and two s8h T-DNA mutant lines (s8h-1 and s8h-2) grown in Fe-depleted hydroponic solution (0 µM Fe²⁺). After 3 weeks of growth in optimal hydroponic solution (40 µM Fe²⁺), plants were transferred to (A) freshly made Fe-depleted solutions and cultured until the chlorotic phenotype was clearly visible. Hydroponic solutions were fully changed once per week. (B) Chlorophyll (Chl) a+b concentration and a/b ratio of wild-type and mutant plants. (C) Relative levels of scopoletin, scopolin, and umbelliferone accumulated in the plant roots. Metabolite profiling of coumarins was done by UHPLC. The results of one representative experimental replicate are presented. Data are presented as mean ±SD from six biological replicates. *P<0.05, **P<0.01.