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DNA sequence of PAT1 intron 1 and derivatives. The sequence of the Columbia wild-type PAT1 intron 1 is shown, and the modifications to introduce a PstI site and to maintain reading frame are indicated. Adjacent exon sequences are in open boxes, PstI sites are underlined, codons are delineated by ticks, and putative branchpoint sequences are shaded. The 5′ splice site point mutation and the extent of the various deletions tested are indicated.
