Table I.

Summary of transgene expression and intron splicing

PAT1:GUS Fusion	No. of Linesa	Relative PAT1:GUS mRNA Accumulationb		Percent Splicedc
		Mean	Range	Mean	Range
Intronless fusions
pAR254	4	1.2	0.8–1.5
pAR281 (+ PstI site)	2	1.6	0.9–2.2
With PAT1 intron 1
pAR253	2	4.6	4.2–4.8	94.9	94.4–95.3
pAR282 (+ PstI site)	1	5.6		95.6
pAR284 (In-frame)	2	4.0	3.9–4.1	93.2	93.1–93.3
Spliceable introns
Δ3-29	3	3.4	3.0–4.0	40.1	31.6–48.6
Δ20-46	6	5.1	3.8–6.6	84.2	80.2–90.0
Δ41-73	4	5.9	4.5–8.1	88.0	86.7–89.7
Δ73-102	2	4.4	4.0–4.9	85.7	82.8–88.6
Unspliceable introns
Reverse	2	0.6	Both 0.6	<2	Both <2
5′ Splice site	4	3.0	2.6–3.3	<2	All <2
Δ6-53	1	2.6		38.5d
Δ55-102	4	4.5	3.5–6.0	<2	<2–2.5

^aThe number of independent, homozygous, single-copy lines tested for each transgene. 

^bThe average amount of GUS-hybridizing mRNA in each line relative to pAR254 line B4, corrected for variations in loading using the endogenous PAT1 mRNA signal. 

^cThe average proportion of RT-PCR product derived from spliced mRNA. 

^dAberrantly spliced.