Table 2.

Expression of P. aeruginosa exoS and exoT genes using q-PCR after exposure to human corneal epithelial cell lysates, TSB (negative control) or TSBi (T3SS-inducing medium) for 16 h. Gene expression was expressed as the mean ± SD relative to PAO1 in TSB.

P. aeruginosa		Relative gene expression mean ± SD
genotype	Conditions	exoT	exoS
PAO1	Lysate	33.83 ± 1.27*	68.73 ± 1.39*
	TSB	1.00 ± 0.07	1.00 ± 0.07
	TSBi	590.10 ± 0.81	342.25 ± 32.33
ΔexoS	Lysate	35.00 ± 3.58*	0
	TSB	0.82 ± 0.01	0
	TSBi	553.00 ± 36.26	0
ΔexoT	Lysate	0	127.52 ± 4.79*,**
	TSB	0	2.15 ± 0.03
	TSBi	0	305.13 ± 19.74

*Significantly lower than that in TSBi, P < 0.0001, Student's t-Test.

**Significantly higher than lysate induction in PAO1, P < 0.0001, Student's t-Test.