Figure 3.

Arsenite exposure effects on HMGB1, MDM2, and TP53 expression. After 7 weeks chronic exposure (A) Western blot and quantitation showing no change in HMGB1. B, Western blot and quantitation showing increased expression of MDM2 isoform A (MDM2-A, 60 kDa), but not isoform MDM2-FL. C, Western blot and quantitation showing both TP53 and TP53 phosphorylated at serine 15 (TP53-S15P) were induced with arsenic exposure. D, Western blot and quantitation showing TP53 hypoacetylated at lysine 382 (TP53-K382Ac) in arsenite exposed cells. Proteins levels were normalized to β-actin levels and means ± SD are plotted. Student’s t-test was used for statistical analyses. *p-value ≤ .05. Samples from 4 independent cultures incubated without arsenite for 7 weeks (lanes 1–4) were compared with samples from 4 independent cultures incubated in parallel with 100 nM NaAsO₂ for 7 weeks (lanes 5–8). After probing for query proteins (HMGB1, MDM2, TP53), blots were stripped and probed for β-actin.