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. 2018 Feb 13;141(4):1063–1074. doi: 10.1093/brain/awy010

Figure 6.

Figure 6

Culture of ex vivo memory and naïve B cells in isolation. Gating strategy to purify switched memory B cells (CD19+CD27+IgD−, light blue), unswitched memory B cells (CD19+CD27+IgD+, dark blue) and naïve B cells (CD19+CD27−IgD+, purple) from six patients with high levels of serum AQP4-IgGs (A; Patients A–F). After culture with mCD40L plus R848, IL-2, TNFα, IL-1β and IL-21) or with ex vivo activated autologous CD4 T cells, supernatants showed AQP4-IgG production (black boxes) from post-germinal centre (GC, three of six patients) and pre-germinal centre (four of six patients) B cells (B). IgG production in culture was measured for the three cell subsets (mean plus standard deviation): naïve = 654 ng/ml ± 280; unswitched memory = 1562 ng/ml ± 957; switched memory = 12 568 ng/ml ± 2098.