Figure 5.

The effect of TGFβ on MMP2 and MMP9 expression in AA-derived PCa cells. E006AA and E006AA-HT cells were treated with TGFβ1 and -β3 in the presence or absence of TGFβRII inhibitor (LY2157299) for 24 h. (A) RT-PCR and (B) qPCR analysis of MMP2 and MMP9 mRNA expression and (C) western blot analysis of MMP2 and MMP9 protein levels in AA-derived PCa cells. (D) Densitometry analysis of protein levels normalized to α-Tubulin. Data represent at least three independent experiments. ‘a–d’ denote significant differences (P < 0.001) among different treatment groups.