Fig. 3.
Root to shoot transport in 21-day-old seedlings of IR29 and Pokkali, grown and measured at their cultivar-specific EC50 values of 35 mM and 50 mM [Na+]ext, respectively, and in response to 0 or 1.67 mM Si. (A) Shoot PTS concentration, (B) transpiration, (C) bypass flow, (D) shoot 24Na+ accumulation normalized to root fresh weight (RFW), and (E) shoot 24Na+ accumulation normalized to shoot fresh weight (SFW). Plants were exposed to PTS or 24Na+ for 24 h before desorption and harvesting. PTS fluorescence is expressed relative to the fluorescence of the loading vessel. Different lower case letters (a, b, c, d) indicate significant differences between conditions (t-test, P<0.05). Data are represented as the mean ±SEM (n=3–7). Empty bars with coloured borders, 0 mM Si; Filled bars with black borders, 1.67 mM Si. Measurements taken at 30 °C in seedlings grown under a 30/20 °C (day/night) temperature cycle.