Figure 1.

Diagrams of the experimental protocols. Control and NaAsO₂-treated HepaRG cells NaAsO₂ were maintained in 14 days growth media for and then for additional 14–28 days in the differentiation media. In Experiment 1: Nondifferentiated HepaRG cells, 3 days after initial seeding, were continuously treated with 1 μM NaAsO₂ for 25 days. The cells were collected after 28 days from initial seeding for the subsequent analyses. In Experiment 2: Nondifferentiated HepaRG cells, 14 days after the initial seeding, were treated with 1 μM NaAsO₂ for additional 14 days. The cells were collected after 28 days from initial seeding for the subsequent analyses. In Experiment 3: Differentiated HepaRG cells, 28 days after the initial seeding, were treated with 1 μM NaAsO₂ for additional 14 days and then collected for subsequent analyses. Control HepaRG cells were maintained in NaAsO₂-free media in all 3 Experiments.