Figure 5.

Whole-genome microarray transcriptomic analysis and summary of affected molecular pathways in the HepaRG cells treated with NaAsO₂. A, Heat maps and unsupervised hierarchical cluster analysis of log-normalized intensities illustrating differences in global gene expression profiles between control HepaRG cells and HepaRG cells treated with NaAsO₂ in Experiments 1–3. Color bar identifies overexpressed (red) and downregulated (blue) genes. B, Principal component analysis illustrating transcriptomic differences between control and NaAsO₂-treated HepaRG cells. C, Venn diagram illustrating a number of differentially expressed genes in Experiments 1–3 in NaAsO₂-treated HepaRG cells as compared with control cells. D, Pathway analysis of differentially expressed genes in HepaRG cells treated with NaAsO₂. Z-scores were calculated using IPA to predict activation (red) or inhibition (blue) of biological functions.