Fig 5. CD4⁺ and CD8⁺ T-cells recruitment into genital tracts of infected wild-type and TLR3^-/- mice.

A) Groups of C57BL/6 and TLR3^-/- mice were infected intravaginally with 1×10⁵ C. muridarum. At day 7 and day 21 post-challenge, reproductive tracts from each group of mice were harvested, and lymphocytes were isolated as described in Materials and Methods. FACS plots showing percentage of CD4⁺ and CD8⁺ cells within the CD3⁺ T-cell population (gated on CD3⁺CD4⁺ or CD3⁺CD8⁺) in the genital tract of a mouse from each group. FACS data showing representative results from each group of mice at days 7 and 21 of chlamydial infection. B) The percentages of CD4 and CD8 T-cells from each group were summarized in the graphs. Data shown are representative results from two independent experiments with three to five mice per time point. Bar graphs show mean number ± SEM. * indicate P values of <0.05. C) Immunohistochemical staining of lymphocytes with antibodies to CD4 or CD8 in the genital tract of an infected mouse at day 21 of infection. Brown stain indicates CD4 or CD8 positive cells. Original magnification ×200.