Fig 5. Phenotyping of the evolved strains.

Strain phenotyping was performed using a high-throughput micro-cultivation instrument, Bioscreen C (Oy Growth Curves Ab Ltd, Helsinki, Finland). Turbidimetric readings were recorded every 20 minutes for 168 hours. Strains were grown at 25°C in rich medium, YPD (0.5% yeast extract, 1% peptone, 2% glucose, pH 6.2). The plots show average growth data from duplicates experiments. The experiments were carried out for 168 hours although we only show the lag and exponential phases of growth on these plots. A) D. anomala (Y863), B) B. custercianus (Y893), C) D. bruxellensis (Y879), D) K. nonfermentans (Y1057), and E) T. pretoriensis (Y1055). C. glabrata could not be tested using the same facilities as the species is an opportunistic pathogen. Phenotypes of L. thermotolerans (Y688) are not reported in this work, whereas L. kluyveri (Y057) has been comprehensively described elsewhere [16].