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. Author manuscript; available in PMC: 2018 Apr 6.
Published in final edited form as: Cell Rep. 2016 Nov 15;17(8):2101–2111. doi: 10.1016/j.celrep.2016.10.054

Figure 2. Non-CG Methylation.

Figure 2

(A) Example of mCH exclusion from lamina-associated domains (shaded areas) in a naive T cell, chr10:100,600,000–124,600,000. x axis, genomic location; y axis, non-CG methylation level (negative values, minus strand; positive values, plus strand). CpG islands (vertical blue bars) and CTCF-binding sites (red triangles) are shown.

(B) Information content in the sequence context of mCH in naive T cells. The methylated cytosine is at position 6.

(C) Conversion rate and mCH. After exclusion of under-converted outliers (red, top plot), the fraction of methylated non-CG cytosines is not influenced by conversion rate (bottom expanded plot), as determined using unmethylated spiked-in bacteriophage DNA. mCH is computed as the fraction of non-converted non-CG cytosines.