Development occurs in the order indicated in the legend, with UHP being the least differentiated cell type.
(A) Methylation surrounding 23,710 constitutively occupied CTCF-binding sites shows oscillations that increase in amplitude as lymphocytes develop.
(B–D) CTCF-binding sites specifically occupied in (B) lymphoblastoid cell line, (C) stem cell line (unoccupied in B cells), and (D) skin cell line (unoccupied in B cells). GC, germinal center B cell; PC, plasma cell; UHP, uncommitted hematopoietic progenitor. See also Figures S2 and S3.