Skip to main content
. 2000 Feb;122(2):543–552. doi: 10.1104/pp.122.2.543

Figure 2.

Figure 2

SDS-PAGE analysis of purification steps of tomato extracellular phosphodiesterase. Lane 1, Molecular mass markers; lane 2, total proteins of the spent medium of a 4-d-old −Pi culture (4 μg of protein); lane 3, pooled active fractions from Sephadex SP-C25 ion-exchange chromatography (4 μg of protein); lane 4, pooled active fractions from the chromatofocusing step eluted at pH 8.1 (3 μg of protein); lane 5, pooled active fractions from Sephadex G-100 gel permeation chromatograhy (1 μg of protein).