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. Author manuscript; available in PMC: 2019 Jan 1.
Published in final edited form as: Cell Signal. 2017 Sep 23;42:44–53. doi: 10.1016/j.cellsig.2017.09.017

Fig. 5.

Fig. 5

Akt-dependent Hif1α regulates mesangial cell hypertrophy. (A and G) Mesangial cells were treated with 0.1 μM JNJ (panel A) or 1 μM MK 2206 (panel G) for 1 h followed by incubation with normal glucose (NG) or high glucose (HG) for 24 h. The cell lysates were immunoblotted with Hif1α and actin antibodies. (B, H and I) Mesangial cells were transfected with dominant negative HA Akt K179M (panel B) or siPDGFRβ (panel H) or PDGFRβ mutant (Y740/751F) (panel I). The transfected cells were incubated with normal glucose or high glucose. The cell lysates were immunoblotted Hif1α, HA, PDGFRβ or actin antibodies as indicated. (C–F) Mesangial cells were transfected with siHif1α or scramble (panels C and D) or HA Hif1α expression vector (panels E and F). The protein synthesis and hypertrophy were determined as described in the Materials and methods section [22,25]. Mean ± SE of 3–6 measurements is shown. *p < 0.01 or 0.001 vs NG; **p < 0.01 vs HG. The bottom panels show the expression of Hif1α.