Table 1. Used primer pairs to detect species-specific eDNA.
| Target species | Primer name | Sequence (5’-3’) | Amplicon size (bp)a | Publication |
|---|---|---|---|---|
| Asellus aquaticus | Aaq-L3 | GGCAATGACCAGATTTACAATGTAA | 147 | Herein |
| Aaq-R3 | ATTTATACGAGGAAATGCTATATCTGG | Herein | ||
| Gammarus pulex | Gpu-L9 | CTCTAACCCTTCTACTTATAAGTAGTA | 179 | Herein |
| Gpu-R11 | GTAGAGATAAAATTAATAGCGCCG | Herein | ||
| Potamopyrgus antipodarum | NZMSF | TGTTTCAAGTGTGCTGGTTTAYA | 89 | Goldberg et al. 2013 |
| NZMSR | CAAATGGRGCTAGTTGGAATTCTTT | Goldberg et al. 2013 |
a Amplicon size is including the primer pair length