Table 1. WNV surveillance plan (sampling and testing) in 2014.

	Lower-risk districts												High-risk districts
Testing of sentinel animals (backyard chickens and horses) for WNV IgG antibodies (ELISA)
Sentinel chickens*	- from 6 settlements per district; - 5 samples per settlement - 4 samplings (1 in June; 1 in July; 1 in August and 1 in September)												- from 10 settlements per district - 5 samples per settlement - 6 samplings (2 in June; 2 in July; 1 in August and 1 in September)
Sentinel horses**	- up to 30 sentinel horses - minimum 3 localities per district - testing of the same horses in three occasions (June-July-August)												- up to 50 sentinel horses - minimum 3 localities per district - testing of the same horses in three occasions (June-July-August)
Real-time RT-PCR detection of WNV in natural reservoirs and vectors
Wild birds	- up to 50 found dead wild birds from May—October												- all found dead throughout the year, or - up to 100 samples of purposely shot or live trapped susceptible bird species from May to October
Mosquitoes (Culex pipiens)	- collection at monthly intervals - 5 localities per district - 5 samplings from May to September												- collection at 2-week intervals - 10 localities per district - 7 samplings from May to September
Sampling strategy
Sampling distribution	Jan	Feb	Mar	Apr	May	Jun	Jul	Aug	Sep	Oct	Nov	Dec	Jan	Feb	Mar	Apr	May	Jun	Jul	Aug	Sep	Oct	Nov	Dec
Chickens
Horses
Mosquitoes
Wild birds

* Surveillance of rural, backyard chickens was limited to chickens hatched in 2014

** Sentinel horses were limited to horses previously tested negative for WNV IgG antibodies