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. 2018 Jan 5;7(4):e1415687. doi: 10.1080/2162402X.2017.1415687

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Figure 6. — DEPDC1-LP2 and MPHOSPH1-LP1 induced cross-priming of CTLs in vivo. HLA-A2 or HLA-A24 Tgm were immunized with DEPDC1-LP2 or MPHOSPH1-LP1 emulsified in IFA. After vaccination, CD8⁺ T cells isolated from the inguinal lymph nodes were stimulated ex vivo with BM-DCs pulsed with DEPDC1-A2_302–311 (A), DEPDC1-A24_294–302 (B), MPHOSPH1-A2_282–291 (C), MPHOSPH1-A24_278–286 (D), or HIV-A2 or -A24 SPs. The number of IFN-γ-producing murine CD8⁺ T cells was analyzed by ex vivo ELISPOT assays. Representative data from three independent experiments (two or three mice in each group) performed in duplicate or triplicate are shown.