Figure 6.

S100A4 inhibits CD8⁺ T cell apoptosis though the Akt signaling pathway. (A) CTLL-2 cells, a CD8⁺ murine lymphoid cell line, were cultured with or without S100A4 (1 µg/mL), or S100A4 combined with anti-S100A4 blocking mAb (6 µg/mL) for 3 d and then subjected to FACS analysis of cell apoptosis. **p < 0.01. (B) Western blots showing the expression of cleaved caspase-3 and caspase-9. (C) CTLL-2 cells were stimulated with S100A4 for varying lengths of time, and the indicated signaling pathways were analyzed by Western blot. (D) An Erk inhibitor or Akt inhibitor was applied to the CTLL-2 culture system supplied with S100A4, and the percentage of apoptotic cells was analyzed via FACS 3 d later. Statistical data are shown (E). **p < 0.01.