Figure 5. Deletion of NOTCH pathway transcriptional mediator Rbpj in POU1F1 lineage has no effect on GH levels.
(A) Growth curves of Pou1f1-Cre;Rbpjfl/fl animals. Male (M) and female (F) mutant (T) and wild-type (NT) littermates were monitored from weaning at 3 weeks of age until 14 weeks of age. There is no significant difference between wild-type littermate and mutants (n = 3 to 9 mice/sex and genotype, mixed-effects model of weight versus age was used, using genotype as fixed factors and subject (mice) as random factors, with analysis of variance (ANOVA) to test the overall effect of genotype on growth, followed by Tukey post-hoc tests). (B, C) GH pituitary contents were assayed by sandwitch ELISA in 6 week-old (B, n = 3 to 6 pituitaries/sex and genotype) and 14 week-old (C, n = 3 to 7 pituitaries/sex and genotype) animals. There is no significant difference between wild-type and mutant animals (unpaired t test performed).