Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| strain, strain background (mus musculus) |
Nkx3.1Cre | Lin, Y., Liu, G., Zhang, Y., Hu, Y.P., Yu, K., Lin, C., McKeehan, K., Xuan, J.W., Ornitz, D.M., Shen, M.M., et al. (2007). Fibroblast growth factor receptor 2 tyrosine kinase is required for prostatic morphogenesis and the acquisition of strict androgen dependency for adult tissue homeostasis. Development 134, 723–734. |
Nkx3-1tm3(cre)Mms | |
| strain, strain background (mus musculus) |
Sox2CreERT2 | Arnold, K., Sarkar, A., Yram, M.A., Polo, J.M., Bronson, R., Sengupta, S., Seandel, M., Geijsen, N., and Hochedlinger, K. (2011). Sox2(+) adult stem and progenitor cells are important for tissue regeneration and survival of mice. Cell Stem Cell 9, 317–329. |
Sox2tm1(cre/ERT2)Hoch | |
| strain, strain background (mus musculus) |
Pou1f1-Cre | this paper | (nogene)Tg(pou1f1-cre)1Rsd | |
| strain, strain background (mus musculus) |
Rbpjfl | Han, H., Tanigaki, K., Yamamoto, N., Kuroda, K., Yoshimoto, M., Nakahata, T., Ikuta, K., and Honjo, T. (2002). Inducible gene knockout of transcription factor recombination signal binding protein-J reveals its essential role in T versus B lineage decision. Int Immunol 14, 637–645. |
RBPJtm1Hon | |
| strain, strain background (mus musculus) |
Rosa26ReYFP | Srinivas, S., Watanabe, T., Lin, C.S., William, C.M., Tanabe, Y., Jessell, T.M., and Costantini, F. (2001). Cre reporter strains produced by targeted insertion of EYFP and ECFP into the ROSA26 locus. BMC Dev Biol 1, 4. |
Gt(Rosa26)Sortm1(EYFP)Cos | |
| strain, strain background (mus musculus) |
Rosa26floxSTOP-Nicd1 | Murtaugh, L.C., Stanger, B.Z., Kwan, K.M., and Melton, D.A. (2003). Notch signaling controls multiple steps of pancreatic differentiation. Proc Natl Acad Sci U S A 100, 14920–14925. |
Gt(Rosa26)Sortm1(Notch1)Dam | |
| strain, strain background (mus musculus) |
Rosa26floxSTOP-Nicd2 | Fujimura, S., Jiang, Q., Kobayashi, C., and Nishinakamura, R. (2010). Notch2 activation in the embryonic kidney depletes nephron progenitors. J Am Soc Nephrol 21, 803–810. |
Gt(Rosa)26Sortm1(Notch2)Nis | |
| strain, strain background (mus musculus) |
Pomc-Cre | Langlais, D., Couture, C., Kmita, M., and Drouin, J. (2013). Adult pituitary cell maintenance: lineage-specific contribution of self-duplication. Mol Endocrinol 27, 1103–1112. |
||
| Antibody | goat anti-Sox2 | ISS | GT15098 | 1/300 |
| Antibody | rat anti-GFP | Fine Chemical products | 04404–84 | 1/1000 |
| Antibodies | anti pituitary hormones |
NHPP | ||
| Antibody | mouse anti-Pax7 | DSHB | P3U1 | 1/100 |