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activated NK cells infiltrate the tumor spheroid at low e:t ratios. Time lapse of the central layers of CRC spheroids (green) obtained by confocal microscopy during the aNK (red) in vitro co-culture at 3:1 e:t ratio. After 24h in co-culture, aNK cells are able to infiltrate the tumor spheroid. This infiltration is observed till the 4th day in which NK cells tend to migrate towards the periphery.
