Figure 3.

A, Precipitation of β-glucosidase activity from K55 (wild-type) maize extract by H95 (null) extract after incubation and centrifugation of extract mixtures at the indicated volumes. Shown are activity in the mixture before centrifugation □; activity after centrifugation, ⊙; and activity in the suspended pellet ▵. Note that the highest precipitation of activity occurs at two to three parts of K55 (wild-type) to five parts of H95 (null extract), and the activity lost from the supernatant is recovered from the pellet. B, β-Glucosidase activity profile of extracts after mixing a fixed volume of H95 (null, 0.5 mL) extract with varying volumes of K55 (wild-type, 0–1.0 mL). Shown are activity in the mixture before centrifugation, ●; activity after centrifugation ⊙; and activity in the suspended pellet ▵. C, Control (K55 extract). Again note that the highest precipitation of enzyme activity occurs at two to three parts of K55 (wild-type) to five parts of H95 (null extract), and the activity lost from the supernatant is recovered from the pellet.