Figure 3. Circulating aggregated amylin increases myocyte Ca²⁺ transient amplitude.

(A–B) Ca²⁺ transients recorded in myocytes from control AKO mice, AKO mice that were infused with aggregated human amylin (h-Amy) and AKO mice injected with monomeric rat amylin (r-Amy). Panel (A) shows recording in myocytes from males and panel (B) shows myocytes from females. (C) Mean Ca²⁺ transient amplitude in cardiac myocytes from male AKO mice infused with either aggregated human amylin (24 cells, 6 mice) or rat amylin (35 myocytes from 6 mice), and control AKO males (15 cells, 3 mice). (D) Mean Ca²⁺ transient amplitude in cardiac myocytes from female AKO mice infused with aggregated human amylin (29 cells, 6 mice) or rat amylin (32 cells, 6 mice) and control AKO females (14 cells, 3 mice).