BOO-induced decrease in nerve density is produced by both a decrease in nerve number and an increase in bladder area, both of which are blocked by glyburide or anakinra. A.) Total number of nerves in a transverse bladder section. B.) Area (in µm2) of the region of interest in a transverse bladder section, as defined in the Materials and Methods section and illustrated in Figure 1. Data are presented as mean ± SEM. N = 4 for Control, 6 for Sham, 6 for BOO + Veh, 5 for BOO + Gly, 4 for BOO + Ana. * indicates P<0.05, ** indicates P<0.01 for the comparisons indicated. Any between-group comparisons that are not indicated were not significant.