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. 2018 Feb 7;29(4):711–722. doi: 10.1007/s13361-017-1886-6

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© The Author(s) 2018

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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MS ion images representing spatial distributions of the identified taurine-conjugated bile acids at m/z 498.2904 ± 0.05 (TCDCA), m/z 514.2836 ± 0.05 (TCA/TMCA), and m/z 530.2789 ± 0.05 (TTHBA) for the whole section of mouse liver at low spatial resolution (pixel size, 70 μm) (a), and in smaller sub-regions of an adjacent tissue section at high spatial resolution (pixel size, 25 μm) (b–d). All ion images were normalized to the 9-AA matrix signal. The green arrows indicate gall bladder (a) or bile vessels (b–d), whereas the red arrows show blood vessels. Scale bars: 5 mm (a), 1000 μm (b, c), and 500 μm (d)