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. 2018 Feb 7;29(4):711–722. doi: 10.1007/s13361-017-1886-6

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© The Author(s) 2018

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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MALDI-MSI single pixel mass spectra from three regions of liver tissue section with different microstructures and chemical compositions: biliary tree (bile duct), blood vessel, and liver parenchyma. The m/z values used for RGB MS images are highlighted in the spectra (a). RGB ion images representing three different compounds, showing clear distinction between anatomical features (as shown by the H&E stained images) across the whole tissue section (pixel size, 70 μm) (b). Expansion of the smaller sub-region (c) as well as smaller sub-regions from an adjacent section (pixel size, 25 μm) (d, e). Scale bars: 5 mm (b), 1000 μm (c, d), and 500 μm (e)