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. 2015 Jun 5;1:1–5. doi: 10.1016/j.biopen.2015.05.001

Fig. 2.

Fig. 2

Effects of CIT in vivo (A) or ex vivo (B) on the expression of leptin, adiponectin and inflammatory marker genes in RET WAT explants from rats. (A) Leptin, adiponectin, F4/80 Cd68, Il-6, TNF-α, NOS II, ASS and ASL mRNA levels in RET WAT explants from 25-month-old CD rats fed the CIT supplemented diet for 3 months were evaluated by RT-qPCR as described in materials and methods. Results were normalized to RPL13 mRNA and expressed in % of control. Control values (100%) were obtained from 25-month-old CD rats in the absence of CIT. Results represent the mean ± SEM of independent experiments (n = 7) carried out in triplicate from different explants. (B) Leptin and adiponectin mRNA levels in RET WAT explants from 2-month-old CD rats, 4-month-old CD rats, 4-month-old HFD rats and 25-month-old CD rats incubated without or with 2.5 mmol/L CIT, as indicated, for 24 h. mRNA levels were evaluated by RT-qPCR as described in materials and methods. Results were normalized to RPL13 mRNA and expressed in % of control. Control values (100%) correspond to those obtained in untreated explants from rats of the corresponding age. Results represent the mean ± SEM of independent experiments (n = 7) carried out in triplicate from different explants. ∗P < 0.05 vs. Control; ∗∗P < 0.01 vs. Control.