Fig. 1.

Effect of heat stress (47 °C) on growth of E. coli BL21(DE3)pLysS cells expressing different variants of wheat cyclophilin TaCypA-1. The cells transformed with non-recombinant pET-28a(+) were used as control. The growth of cultures was monitored by recording absorbance at 600 nm (A) and by determining the colony forming units (cfu/ml) (B). The starting viable cell density was similar for all cultures. Data represent mean ± S.E of three independent biological replicates. Asterisks represent level of significance determined after two way analysis of variance. ** = statistically significant at P < .001 (LSD = 0.050); * = statistically significant at P = .011 at 2 h and P = .001 at 3 h and 5 h (LSD = 0.050).