Fig. 3.

Effect of CsA (1 μM) on growth of E. coli BL21(DE3)pLysS cells transformed with wheat cyclophilin TaCypA-1 gene. The cells transformed with non-recombinant pET-28a(+) were used as control. The growth of cultures was monitored by recording the absorbance at 600 nm (A) and by determining the colony forming units (cfu/ml) (B). The starting viable cell density was similar for all cultures. Asterisks represent level of significance determined after two way analysis of variance by using Fischer LSD method. * = statistically significant at P < .001 (LSD = 0.050).