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. 2015 Nov 30;2:9–15. doi: 10.1016/j.biopen.2015.11.003

Fig. 4.

Fig. 4

15% SDS-PAGE analysis of total proteins extracted from the recombinant E. coli cells over-expressing TaCypA-1 (A), TaCypA-1 (22-171) (B) TaCypA-1 (51-171) (C) and TaCypA-1 (71-171) (D) after exposure to heat stress at 47 °C. The E. coli lysates were analysed after induction with 0.5 mM of isopropyl β-d-1-thiogalactopyranoside after 2 h, 3 h and 5 h of growth. The cells transformed with non-recombinant pET28a (+) were used as control. The induced proteins are indicated by arrows. M: molecular weight markers.