Figure 13.

Excitatory responses to EFS are abolished by atropine and NK1 receptor antagonist. A, B, Representative ST maps showing inhibition of Ca²⁺ transients by atropine (1 μM) and RP 67580 (1 μM). These experiments were conducted in the presence of L-NNA (100 μM) in the presence of MRS 2500 (1 μM) during and post nerve stimulation periods (EFS at 10 Hz 5 s; indicated by the red line and dotted white box in ST maps). C–F, Summary data showing the effects of a combination of L-NNA, MRS 2500, and atropine and RP 67580 on Ca²⁺ transient frequency (C), amplitude (D), duration (E), and spatial spread (F) in ICC-DMP during control conditions, excitatory periods during EFS (final 3 s), and in the post-EFS period, n = 5, c = 32; ns = p > 0.05, **p < 0.01, ***p < 0.001.