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. 2016 Mar 2;2:33–40. doi: 10.1016/j.biopen.2016.02.003

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© 2016 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Fig. 3 — Elution profile of EPS_D1 analyzed by HPSEC/RID coupled to a UV–vis detector. Diaporthe sp. JF766998 was grown under submerged culture for 72, 96 and 168 h, and EPS were designated EPS_D1-72, EPS_D1-96 and EPS_D1-168, respectively. Aliquot of EPS injected: 200 μL (1 mg/mL). Gel permeation columns with exclusion limit of 7 × 10⁶, 4 × 10⁵, 8 × 10⁴ and 5 × 10³ Da arranged in series. Flow rate = 0.6 mL/min. Eluent: 0.1 M NaNO₃ with sodium azide (0.03%). UV–vis 280 nm (), RID ().

Inline graphic — Elution profile of EPS_D1 analyzed by HPSEC/RID coupled to a UV–vis detector. Diaporthe sp. JF766998 was grown under submerged culture for 72, 96 and 168 h, and EPS were designated EPS_D1-72, EPS_D1-96 and EPS_D1-168, respectively. Aliquot of EPS injected: 200 μL (1 mg/mL). Gel permeation columns with exclusion limit of 7 × 10⁶, 4 × 10⁵, 8 × 10⁴ and 5 × 10³ Da arranged in series. Flow rate = 0.6 mL/min. Eluent: 0.1 M NaNO₃ with sodium azide (0.03%). UV–vis 280 nm (), RID ().