Fig. 13.

Elution profiles for tobacco leaf rubisco activase contents and activities. Rubisco was isolated from leaves of in vitro grown tobacco plants treated with Cd in addition to the control. Fractionation was done on Q-Sepharose column. The column eluted with BTP (pH 7.2) containing 0.0–0.5 M NaCl gradient. After passing through anion exchanger column fractions were collected and assayed at 280 nm. Closed circles (●) represent protein, open circles (○) represent rubisco activity, whilst the straight line indicates NaCl gradient.