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. 2016 Mar 2;2:41–51. doi: 10.1016/j.biopen.2016.02.002

Fig. 17.

Fig. 17

Elution profiles for tobacco leaf rubisco activase contents and activities. Rubisco was isolated from leaves of in vitro grown tobacco plants treated with Cu + SNP in addition to the control. Fractionation was done on Q-Sepharose column. The column eluted with BTP (pH 7.2) containing 0.0–0.5 M NaCl gradient. After passing through anion exchanger column fractions were collected and assayed at 280 nm. Closed circles (●) represent protein, open circles (○) represent rubisco activity, whilst the straight line indicates NaCl gradient.