Fig. 6.

Elution profiles for tobacco leaf protein and rubisco activities. Rubisco was isolated from leaves of in vitro grown tobacco plants treated with Cu in addition to the control. Fractionation was done on Q-Sepharose column. The column was eluted with BTP (pH 7.2) containing 0.1–0.5 M NaCl gradient. After passing through anion exchanger column fractions were collected and assayed at 280 nm. Closed circles (●) represent protein, open circles (○) represent rubisco activity, while the straight line indicates NaCl gradient.