Fig. 8.

Elution profiles for tobacco leaf protein and rubisco activities. Rubisco was isolated from leaves of in vitro grown tobacco plants treated with Cd + SNP in addition to the control. Fractionation was done on Q-Sepharose column. The column was eluted with BTP (pH 7.2) containing 0.1–0.5 M NaCl gradient. After passing through anion exchanger column fractions were collected and assayed at 280 nm. Closed circles (●) represent protein, open circles (○) represent rubisco activity, while the straight line indicates NaCl gradient.