Fig. 2.

Changes in IL-8 expression in MG63 and hMSCs induced by the co-culture condition and rIL-8 administration. a Changes in IL-8 expression in MG63 were assessed by qRT-PCR. The co-culture and rIL-8 addition significantly increased the expression of mRNA of IL-8. (**) p < 0.01. b Changes in IL-8 expression in hMSCs were assessed by qRT-PCR. The co-culture and rIL-8 addition significantly increased the expression of mRNA for IL-8. (**) p < 0.01. c Changes in IL-8 protein expression of intra-cellular hMSCs and MG63 were assessed by western blot analysis. The co-culture and addition of rIL-8 increased the expression of IL-8 protein in MG63 and hMSCs. d The quantification of western blot analysis. Data represents represent the mean ± SD of three independent experiments. p < 0.05 was considered to indicate significance: (*) p < 0.05, (**) p < 0.01