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. 2018 Apr 6;16:13. doi: 10.1186/s12964-018-0225-2

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Effects of neutralizing anti-IL-8 Ab on IL-8 expression in mono-cultured and co-cultured MG63 and hMSCs. a Changes in IL-8 expression in MG63 were assessed by qRT-PCR. The addition of nIL-8 Ab to MG63 decreased the expression of IL-8 mRNA. b Changes in IL-8 expression in hMSCs were assessed by qRT-PCR. The addition of nIL-8 Ab to hMSCs decreased the expression of IL-8 mRNA. c Changes in IL-8 protein expression in hMSCs and MG63 were assessed by western blot analysis. The addition of nIL-8 Ab to MG63 and hMSCs decreased the expression of IL-8 protein in these cells. d The quantification of western blot analysis. Data represents represent the mean ± SD of three independent experiments. p < 0.05 was considered to indicate significance: (*) p < 0.05, (**) p < 0.01